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sonication free protein extraction... - (Sep/30/2005 )

Hi,

I'm looking for a protein extraction protocole based on gently lysis of E. coli cells, without sonication or French press, just detergents, lysozyme and DNAse. I want to use Cellytic from Sigma but I have no idea if it's efficient or no.
Thanks.

-sylva_rob-

I can't give you the brand of this thing as I don't have it right now with me, but there is something called BugBuster (how appropriate) that gives me the same yields as sonication...

-UniSPheryk-

You can find these kinds of protocol on web very easily. Most of them are similary to each other. However, I have tried some, they are not satisfied. I want to try Frech press or some others.

-maryaiyu-