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sonication free protein extraction... - (Sep/30/2005 )


I'm looking for a protein extraction protocole based on gently lysis of E. coli cells, without sonication or French press, just detergents, lysozyme and DNAse. I want to use Cellytic from Sigma but I have no idea if it's efficient or no.


I can't give you the brand of this thing as I don't have it right now with me, but there is something called BugBuster (how appropriate) that gives me the same yields as sonication...


You can find these kinds of protocol on web very easily. Most of them are similary to each other. However, I have tried some, they are not satisfied. I want to try Frech press or some others.