upscaling protein production - is type of vector and host strain crucial factors? (May/08/2008 )
Hi. I'm looking to express an enzyme in E. coli with a view of upscaling the production of the enzyme in the future in perhaps, a bioreactor. As of now I have yet to even start with lab-scale production of the enzyme. I want to know if the type of vector and host strain would be really crucial in an upscaling procedure or is it more dependent on the culture methods, i.e high-cell density fermentation etc.
-khommy-
QUOTE (khommy @ May 9 2008, 03:33 PM)
Hi. I'm looking to express an enzyme in E. coli with a view of upscaling the production of the enzyme in the future in perhaps, a bioreactor. As of now I have yet to even start with lab-scale production of the enzyme. I want to know if the type of vector and host strain would be really crucial in an upscaling procedure or is it more dependent on the culture methods, i.e high-cell density fermentation etc.
Scale-up should be a matter of volume or culture method. If a protein is toxic at a small scale, it will still be toxic at a large volume. Similarly, expression level and solubility should not vary significantly. But remember, you're working with a biological system, so there's always the chance that something weird could happen... ;-)
-swanny-