Protein extraction form gels - (May/14/2001 )
I am looking for a method to extract a protein from a coomassie/ silver stained gel. I am then going to digest it with tryp so I need it as concentrated as possible
Try Elutrap, it is a realy convenient method for electroelution of proteins from SDS-PAGE. But it works better if you don't make a strong staining procedure (try amido black staining).
You can find the description of the method in :http://www.s-und-s.de/Pages-NEU-eng/UB3/Li.../labmanual.html
Good luck !
instead of amido black staining. u can use CuCl2.H2) (0.3M) for negative staining, cut the unstained bands and destain with 0.25M tris and 0.25M EDTA