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need ideas for protein protein interaction - protein interaction assays (Dec/15/2005 )

What's another option for a INDIRECT protein-protein interaction assay besides a CO-Immunoppt? Apparently, yeast-two-hybrid and GST pull down assay are for direct interaction between proteins. Any advice would be appreciated.



you can try to detect co-localization of your proteins by immunofluorescence and confocal microscopy, if you have antibodies available.




you can also try some in situ photo-crosslinking


Maybe a far-western? But that might be too direct..........