Protocol Online logo
Top : Forum Archives: : Molecular Biology

insulin like growth factor IIprotein - (Mar/22/2001 )

does anybody have a general protocol for recursive PCR?I will be using 6 oligo @ 100 bp long to make an insert about 500 bplong.


See Prodromou et al Protein Engineering (1992) 8, 827-9

I believe the most important bit is the oligo design, ~20 bases overlap, check sequence of the overlap ends to make sure they don't prime with other oligos. There should be other references if you do a literature search.You can probably contact the guy himself (Chris Prodromou) if you have more question. He has just moved to the Institute of Cancer Research in London.