Phosphorylation of TP53 - (Nov/28/2005 )

Hallo all,

I'm invetsigating the expression of the TP53 Protein in human lymphocytes. I detect the protein with the DO-1 Antibody from Santa Cruz. For protein separation I use the NuPAge BIS-Tris Gels 10%. On my blots appear two bands of just above 50 kDA. Is ist possible that the second band would be the phosphorylated protein ?

thanks
superawo

hi
i see two tiny bands too. I've supposed that the second one is phosphorylated form of TP53

i think you're both correct. i work with a 58kDa thr/ser phosphorylated cyanobacterial protein and we routinely run it on 10% gels to see the phosphorylated and dephosphorylated forms. the phosphorylated protein runs just a little above the unphosphorylated form.


this paper has some good pics of a phos/dephos protein run on a gel:

Nishiwaki T. et al "Role of KaiC phosphorylation in the circadian clock system of Synechococcus elongatus PCC 7942" PNAS 2004 Sep 21;101(38):13927-32

hope that helps!