How resuspend protein after (NH4)2SO4 precipitation? - (Nov/09/2004 )

Hi all,

I usually precipitate my protein sample with ammonium sulfate but after centrifugue,it is very difficult to resuspend it in a buffer such as PBS since the precipitate remains very hard in the bottom of the centrifugue tube.

Does anyone know any way to do this more easily?

Thanks in advance

-celvas-

QUOTE (celvas @ Nov 9 2004, 01:10 AM)

Hi i also do ammonium sulphate ppt for my protein and I resuspend it in sodium phospahate buffer and I don' have any problem about resolving them.Do you study in small amounts?I resuspend by pipeting very slowly in 50-100 microliter buffer usually and how long do you centrifuge?

-BURCU:)-

Thanks for your reply Burcu,

I centrifugue about 50 mL each tube for 15 minutes at 15000 rpm in a Sorval SS-34 centrifugue.

-celvas-