Protein pellet solubilization trouble - (Jun/16/2005 )
i've used a tca precipitation with nadoc and acetone, but after drying i cannot resuspend my pellet with my buffer lysis. i don't know if the problem is tht my samples have been in tca at 4ºc overnight. it's too much?
make an other wash with cold acetone. after this, let dry for at least 30' and resuspend in your lysis buffer, preheated and incubate at 37° with vigourous shaking.
if not working, let mix overnight at 4°
this topic may help too.