red fluorescence protein - (Nov/23/2006 )
Does a fluorescent protein looses its fluorescence when denatured?
Can we detect fluorescence of a protein by running SDS gel or native gel?
-minnee-
QUOTE (minnee @ Nov 23 2006, 11:03 PM)
Does a fluorescent protein looses its fluorescence when denatured?
Can we detect fluorescence of a protein by running SDS gel or native gel?
Can we detect fluorescence of a protein by running SDS gel or native gel?
Q1: yes, fluorescence depends on protein conformation
Q2: never tried, but should fail in SDS; native gel may succeed after an appropriate re-activation protocol
-The Bearer-