protein G, binding buffer pH? - (Feb/06/2008 )

hi all,

wat is the pH of binding buffer in protein G mediated antibody purification.
is it acidic or neutral or basic?


thanks in advance.

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According to the technical information from Oncogene research products, the pH of the binding buffer is 7... which is neutral.

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thanks

i found pH-5 for binding buffer for recombinant protein G from PIERCE. how does it differ between company to company?

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Most companies have done QC on their products and will recommend the use of certain buffer at certain pH.
Therefore, if you use protein G from PIERCE, then follow their recommendation and use binding buffer at pH5.
Or you may wish to find the optimum pH for your specific antibody.

Hope this may help.

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