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Protein with His6 tag - (Mar/16/2005 )

Hi all,
my question is which is the most successful method for purification of protein with His6 tag?
I have tried with Ni-NTA Spin Kit (QIAGEN) but i'm not so satisfied...i loosed a lot of protein during the purification steps and that (as usual) i received multi band during elution step...
What about magnetic beads?
Thanks in advance!


during a practice, i've sucessfully use beads. It's an easy method which give good results. just swirling and magnetic field....


Ni column usually is the preliminary purification step, you can use other column like ion exchange or gel filtration to further purify your protein