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protein storage following purification - (Apr/25/2006 )

I'm looking for information on buffers to use for storing purified protein. I'll be purifying large quantities of a kinase for crystallography, so I'll either need to store small frozen aliquots or preferably a couple larger vials with glycerol. I would like to have some anyway in glycerol at -20 for assays and such.

I've had trouble finding info on buffer recipes. I'm particular concerned about the samples for crystallography as I don't what to store the protein in something that will cause problems with crystal formation.

Is it all just trial and error or are there some hard and fast quidelines?

-jaknight-

you can store ur protein at -70 in same buffer in which it is, by simple aliquating , the amount which u need for crystalliztion for a single time., because freezing and thawing will lead to degradation. being a crystallographer my exp. is not very good with freezed protein
all the best

-sarvind-