Purification after oligos phosphorylation? - (Dec/06/2006 )
Hi to all!
I want to insert one 55 bps to one 8 kbs vector.
I want to phosphorylate my oligos before annealing. Will I have to purify them before ligation to dephosphorylated and purified vector?
-Goliadkine-
You can purify it by column or Qiagen kit, or You might simply heat 65C for 20 mins to inactive phosporylation before add to ligation.
while, I actually dislike the idea to phosphorylated such short insert by myself, synthezed oligos with 5' phosphorylated is more efficient!
-rye-