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Purification after oligos phosphorylation? - (Dec/06/2006 )

Hi to all!

I want to insert one 55 bps to one 8 kbs vector.

I want to phosphorylate my oligos before annealing. Will I have to purify them before ligation to dephosphorylated and purified vector?

-Goliadkine-

You can purify it by column or Qiagen kit, or You might simply heat 65C for 20 mins to inactive phosporylation before add to ligation.
while, I actually dislike the idea to phosphorylated such short insert by myself, synthezed oligos with 5' phosphorylated is more efficient!

-rye-