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How many ways are there to conc. protein? - (Jul/29/2008 )

Just like the title says, i would like to know the ways to concentrate protein other then ultrafiltration. Thanks

Andrew

-olifu02-

QUOTE (olifu02 @ Jul 30 2008, 05:12 AM)
Just like the title says, i would like to know the ways to concentrate protein other then ultrafiltration. Thanks

Andrew



i use speedivac to concentrate my prot.

-heidi-

You can precipitate either with Ammonium sulfate or Acetone and then resuspend in a smaller volume, you can also use PEG concentration

-Jipes-

you can also lyophilise your protein and resuspend in the volume needed. But you may consider the final salt concentration.

-fred_33-

Vaccum dialysis

-sgt4boston-

You can also use RotaVapor to quickly evaporate the water in your protein solution.

-ionchannelbk-