How to remove nuclease contamination from protein prep? - (Nov/22/2006 )
I just purified a fusion protein, which is very pure as visualized by SDS-PAGE. Unfortunately, it has nuclease contamination. I have tried Heparin and ionic exchange columns, neither is successful. As I have to use nuclease-free protein in my assay, could anyone give me some advice? Thanks a lot.
I used to use streptomycin to eliminate nucleic acids from my proteins extracts. I usually precipitate the crude extract with streptomaycin at 4 C, then I centrifuge at 12,000 rpm. Then using chromatrography yu can elimininate the streptomycin or just with a desalting column,