how to express GST fusion trunctated protein? - GST fusion protein (Feb/11/2008 )

I did the cloning before, but that was for UTR cloning.

I want to know Do I need to clone the start codon into the vector before the truncated gene of interest? Does the vector usually contain the start codon?

Maybe I don't need to clone the stop codon after the gene as I want to express a GST fusion protein?

many thanks in advance.

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You might need to give us a bit more detail. What vector do you want to use? N-terminal GST? C-terminal GST?

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I want to use N terminal GST vector, thanks.

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The N-terminal GST will start the process for you, so you won't need the start codon from your gene. Similarly, the stop codon should be included in the plasmid, especially if it's a commercial expression plasmid. If you have a look at t he vector map, which you should be able to get from the company website, you should hopefully find how many residues after the end of your protein will be added by the vector until its own stop codon is reached. If you really don't want any other residues, you certainly can add a stop to the end of your gene of interest.

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