Non-isotope detection of mRNA and protein interaction - (Dec/29/2005 )

I have a truncated form protein.
I want to know if this protein will interact with mRNA .
are threr any ways that without using radioisotop ?

any suggestions will be great, thanks............

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more details, please?

I would think perhaps some sort of pulldown binding assay...

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thanks for your reply...

what i have : 1. truncated protein
2. Ab against wild type protein
3. mRNA from cell extract(maybe synthesize this protein 5' UTR mRNA)

I haven't done such kind of experiment ,before.
In the literature search, I found the wild type protein is an mRNA binding protein.
they use EMSA to do this experiment.
but I don't want to use radioisotop labelling method.

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can you label RNA with a non-radioactive method? emsa's aren't too bad, once you get started

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