Protocol Online logo
Top : Forum Archives: : Molecular Biology

protein expression - What is a suitable strain (Jul/25/2002 )

hi! i have a gene of interest cloned in the vector pGEM3Z. I would like to know whether it is possible to express the protein in this vector itself ( since it has a promoter region and termination region). If yes what is the suitable strain for expression. can i use BL21 DE3. Do i have to induce expression by adding IPTG for this strain. my gene is under the SP6 promoter.
Thank you in advance for your help.

-Maitreyis-

Usually Bl21 are good for expression of recombinant bacteria.
Are you sure your promoter (Sp6) is a procaryote promoter ? I may do a mistake but I think that sp6 promoter is used for in vitro translation in eucaryote cells.
Usually to expressed protein in bacteria we use plasmid such as PGEX (look in clonetech catalog).

-cecile-