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How to elute protein from SDS-PAGE gels - (Apr/09/2007 )

How to elute proteins from SDS gels, any protocol

-coffee-

you can electroelute.

or

the easiest way is to homogenize the excised band in a little buffer and centrifuge to pellet the gel and collect the supernate.

-mdfenko-

QUOTE (coffee @ Apr 9 2007, 02:22 PM)
How to elute proteins from SDS gels, any protocol


is it fixed? so electroelution should be successful optionally w/o mincing the gel;

-The Bearer-

QUOTE (coffee @ Apr 9 2007, 05:22 AM)
How to elute proteins from SDS gels, any protocol


Take a look here
manual Gebaflex

I hope it helps

-circlepoint-