Protocol Online logo
Top : Forum Archives: : Immunology and Histology

Protein crystals - (Aug/14/2006 )

Hi all,
Please can you help me in this, I got few crystals of protein in thin tube covered with phosphate buffer and I dont know how to get them out to dissolve them in NaCl solution. Shall I transfer them to other vial or I can handle them in their tube? sad.gif

-modelingmaster-

can you pull a fine capillary tube out of a 9" pipet using gas flame, suck the PBS-protein solution out, then place NaCl back to dissolve the crystals?

-genehunter-1-

You sure these are protein crystals and not crystalised phosphate buffer? What do you want to do with your protein? Are you doing crystallography experiments?

-paraboxa-

Yes for sure its protein crystals and I want them in their active form, its an enzyme and I'll use it in inhibition assay. I have only very very small amount and I don't want to lose! sad.gif

-modelingmaster-