isolation of nuclear protein - isolation of a nuclear protein, namely Akt protein (Mar/10/2005 )
I would like to isolate Akt protein, a kinase, from nuclei of cardiomyocytes. Do any of you have experience in this topic ? Please give e.g. a standard protocol for isolation. I should use it at first in western blott.
Thank you for information.
i tink you first have to do total proteins nuclear extract.
You can find a protocol here :
here is a receipe for cytoplasmic and nuclear extract :
finally here the global nuclear extract page from protocol online :