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isolation of nuclear protein - isolation of a nuclear protein, namely Akt protein (Mar/10/2005 )

Hi all,

I would like to isolate Akt protein, a kinase, from nuclei of cardiomyocytes. Do any of you have experience in this topic ? Please give e.g. a standard protocol for isolation. I should use it at first in western blott.

Thank you for information.

Andree rolleyes.gif


i tink you first have to do total proteins nuclear extract.
You can find a protocol here :

here is a receipe for cytoplasmic and nuclear extract :

finally here the global nuclear extract page from protocol online :