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how to check the degradation rate of a specific protein - (Sep/05/2006 )

thanks a lot.


QUOTE (stormlina @ Sep 5 2006, 08:59 PM)
thanks a lot.

14C-Amino acid metabolic labeling and chasing with cold AA afterwards is commonly used. You probably can also get a grip with protein synthesis inhibitors as well. I am not sure how clean that is.


To study protein halflife, cycloheximide is commonly used.