Stability of protein in powdered leaf sample - Protein stability after quick freeze in liquid N2 (Jul/16/2005 )
Stability of protein/enzyme in powdered leaf sample after quick freezing in liquid nitrogen
I am very urgently need to know abt this (i.e) whether the protein/enzyme presnet in plant leaf (sample) will be stable even after powdered in liquid nitrogen and stored at room temperature.
Is so how long they will be stable. What determines the stablity of the protein from plant leaves.
Pls do mail me the details which will really be a timely help.
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Generally protein will be degraded if you grind leaf and let it thaw in room temp. You need to mix the powder with buffer (contain protease inhibitor) immediately.
how long they will be stable? --> no one knows because proteins are all different.