physicochemical characterization of proteins - (Mar/20/2006 )
that's a standard curve. you can use it to determine your protein concentration by its absorbance at 280nm.
Thanks for your reply.
can the curve tell anything about the structure of the protein?
I don't understand why the curve reaches plateau finally.
it's not really a plateau. it is linear only until a certain point (usually from 0.1 to 0.7 A). some of the limitation is caused by the spectrophotometers detector limits.
for the linear part, can I say that there may be a Trp residue on the protein surface?
at 280nm you are reading ring structures in your aa sequence (ie trp, phe, tyr). the more rings the greater the absorbance.