making large proteins - (Jul/13/2006 )
Does anyone have any preferred methods for large protein synthesis(~100kDa) that they would like to share or discuss? If not could anyone direct me to any info on this subject? Thanks.
what type of proteins do u are really interested in?
i use baculovirus system for 150 routinely and up to 250kD production
Thanks for your help, but I can't translate your suggestion into english. Something about pQE30 and pET28 antibodies?