WHat's the difference between Protein A and G? - (Mar/27/2006 )
I have to do some co-IP and I was wondering what's the difference between recombinant proteinA and rec.protein G?
And one more question what's the difference between sepharose and agarose coupling?
Thanks for your help
protein A and G differ in their selectivity for immunoglobulin types (someone will surely come up with a better explanation)
Sepharose is a trademark name for agarose prepared and sold by pharmacia/amersham/ge healthcare. the CL version is crosslinked and therefore more stable.
here you can find the binding profiles of protein A and protein G