Protocol Online logo
Top : Forum Archives: : General Lab Techniques

WHat's the difference between Protein A and G? - (Mar/27/2006 )

Hi everyone

I have to do some co-IP and I was wondering what's the difference between recombinant proteinA and rec.protein G?
And one more question what's the difference between sepharose and agarose coupling?

Thanks for your help


protein A and G differ in their selectivity for immunoglobulin types (someone will surely come up with a better explanation)

Sepharose is a trademark name for agarose prepared and sold by pharmacia/amersham/ge healthcare. the CL version is crosslinked and therefore more stable.


here you can find the binding profiles of protein A and protein G