Problem with expression of DsRed fusion protein. - (Feb/11/2008 )
I have a problem with the expresion of DsRed, my fusion protein seems to aggregate, i want to know if somebody has the same problem and what can i do?
Thanks in advance.
Dsred is not a monomer so it aggregates. i forget if it was a dimer or a tetramer. Try some monomeric fluorescent proteins like acGFP (clontech).
ok, I´ll do it.
...or RFPTag from Evrogen
There is a review from Roger Tsien's lab about different fluorescent proteins. "A guide to choosing fluorescent protiens" You will find it very interesting.
Might be useful