How to quantify fluorescent-tagged protein - (Oct/24/2005 )
one of my colleagues is looking for a method to quantify a protein which has been tagged with a fluorescent tag. The fluorescent tag is covalently linked to protein glucidic chains.
We wonder what should be a suitable method to quantify the fluorescent-tagged protein taking into account that we cannot measure it by spectrophotometry since the fluorescent tag does absorb UV light in a broad range.
We are wondering too if it exist any method to specificly quantify fluorescent-tagged protein from total protein.
Thanks a lot in advance
check the protocol of Molecular probes. They have a labeling kit with which u can label DNA and I think protein (not too sure though)....They describe the way to quantify flourescently tagged molecules.
You maybe able to use a fluorimeter depending upon the fluorochrome that you used.