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[western blotting] Loading control in 6% gel? - Please suggest good loading control protein of high molecular weight (Oct/17/2007 )

Hi All,

Recently I did western blotting using 6% SDS-PAGE gel and I got good result for the protein of my interest (160KD). But actin (~40KD) seems already gone out and I

need another loading control to normalize expression levels. Proteins below ~65KD seem already gone, so please recommend good loading control greater than 65KD.

Thank you all in advance!


you can always use a little antibody smile.gif


Stat 1 (91kDa) is reliable protein for human cells.