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Calculating protein size - how to calculate the protein size when cloned in pet vector (Dec/21/2007 )

I would like to know theriotically how to calculate the protein size cloned in pET28A+ vector along with the His tag.

If my actual protein size is 15KDa, along with the His tag will it be 17-18 KDa.

Please help me

Joey

-joeypostdoc-

enter the whole protein sequence in this website and it will give you the mass.

-scolix-