Hi, there,

Does anybody know that DTT is compatible with BCA protein assay?

---

there are maximum concentrations stated in tech bulletins of each manufacturer -
seems to depend on which kit or reagent you use(???)
At least there are different data with sigma-aldrich and pierce.
Just check on manufactorer's home page...

---

you can check on the provider webpage
http://www.piercenet.com/products/browse.cfm?fldID=02020101

they say yes with 1 mM.
And if I remember well, I would say it's true.

---

According to Pierce, 1mM DTT is compatible with BCA

---