Protein expression changes after freezing+thawing - (Apr/24/2008 )
I made a 293 cell line expressing an empty vector (as control). However, I now found that the expression of at least one protein I am studying changed greatly after freezing in liquid nitrogen and thawing. Is this normal? Did I not do the freezing/thawing properly, or freezing/thawing can do this to cells? The cells appear otherwise healthy and the rate of proliferation is not too different.
of course, freezing and thawing imply some stress to cells, and you may find some differences f.i. in phosphoproteom to compensate stressy moments;
you may take it positively, and find some novel aspects to your protein of interest