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Protein Extraction - (Dec/21/2007 )


Im looking for a protocol to extract proteins from rat liver tissue. I will then be looking at the level of the nuclear transcription factor ERalpha and ERbeta, (estrogen receptor alpha and beta) by western blotting.
I found a protocol with Pierce online regarding their T-per reagent for total protein extraction but im not sure if this is suitable or should i be using a specific nuclear protein extraction protocol such as the one from Qiagen. Or does anybody have an in-house method?
Im just not sure how to go about choosing a buffer, the salts, protease inhibitors etc, specifically needed for this tissue and the protein im looking at, for making an in-house procedure.

Much appreciated



Iam just trying to help I found total Protein Extraction Kit from millipore