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Dialysis doubt - (Oct/17/2007 )

Hi guys
I have doubt about dialyis. Is dialysis can be used to exchange the buffer ? My protein is in Tris Buffer and i want to change it 10 mM Pot phosphate buffer. Can i dialyse the protein sample against the phosphate buffer ? If yes How long i should dialyse?

Thanks for help


dialysis in 500ml of phsophate buffer for 6-8hr, then change buffer and dialysis for overnight, and finally change buffer again and dialysis for a further 6hr.

Hope this may help.

-Minnie Mouse-