How to determine protein concentration - how to determibne particular protein concentration from a tissue homog (Feb/23/2007 )

Hello there

I have a theorectical question...

How do you go about determining the concentration of a particular protein in a tissue homogenate?

Do any of you guys do this and if so how?

If you have an antibody to your protein of interest I guess you could run your sample over a column with the antibody bound to it and determine the concentration of the elution but is there a better way?

Any suggestion would be really appreciated

lab-rat

I vote for ELISA or western.

I vote for ELISA, much more quantitative than western-blot which is more a qualitative analysis