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any software can calculate the charge of protein - (Jan/31/2006 )

as up, any software or website can calculate the protein charge?
I want to use separose column, so need to know wheter m y protein is negative or positive charge.

Thanks all tongue.gif

-cathy-

alot of resource can be found on expasy.org

for calculation of pI you can use this

-Kersten-

QUOTE (cathy @ Jan 31 2006, 04:15 PM)
as up, any software or website can calculate the protein charge?
I want to use separose column, so need to know wheter m y protein is negative or positive charge.

Thanks all tongue.gif


Go to Expasy website you will find many tools for protein including the PI determination wink.gif

Pesji cool.gif

-pesji-

QUOTE (cathy @ Jan 31 2006, 04:15 PM)
as up, any software or website can calculate the protein charge?
I want to use separose column, so need to know wheter m y protein is negative or positive charge.

Thanks all tongue.gif



ExPASy

There you can paste your sequence, get the pI and you can decide, weather you use a more acidic puffer below the pI (protein is protonated) or you want a alkalic buffer for negative charged proteins.

Oli cool.gif

-ms-olli-

hm ... the three answers are kind of the same, or am I wrong blink.gif

-Kersten-

for confirmation you are not seeing in triplicate : expasy is good for calculating pI


http://sosnick.uchicago.edu/pi_tool.html

-fred_33-

you will find couple of bioinfo tools on http://www.infobiogen.fr/

S├ębastien_

-tryptofan-

thanks all
the replys are really helpful.

-cathy-

Just to be different, you can also use PepStats, part of the Emboss package. See here.

-HomeBrew-