Is it OK to use monoclonal antibody against its host protein for westen - (Sep/24/2007 )

Hello, I am new to Western and immunology. I would like to know if it is OK to use monoclonal antibody raised for example in mouse to detect mouse protein in Western blotting.

Thanks in advance for your input.

-predoc-

QUOTE (predoc @ Sep 25 2007, 10:48 AM)

I can't imagine there would be too much trouble; your monoclonal is only going to bind to the specific Ag.

-swanny-

You may have 2nd Ab issue. Biotin and ABC can get around thsi problem.

-genehunter-1-

QUOTE (predoc @ Sep 24 2007, 08:48 PM)

do you have this antibody? or are you trying to find out if it is possible to make this antibody?

it may be difficult to raise an antibody against a protein native to the organism. i guess you would have to mimic an autoimmune disease.

-mdfenko-

during my PhD I raised polyclonal antibodies to a mouse protein in mouse, and they worked really nice in WB and IHC, so I can only imagine a monoclonal should work much nicer

-almost a doctor-