In immunoprecipitation, how to elute proteins without IgG disturb? - (Nov/22/2007 )
hi, everybody! I wanted to use the proteins eluted from immunoprecipitated beads to perform 2D-PAGE. I boiled the beads and used 2D-clean-up kit to remove SDS and other salts, but the IgG was also released from beads and disturbed the 2D gel pattern. who can tell me a method that can effectively elute proteins but not IgG from beads . kit is ok, too. thanks!
You may wish to crosslinking the antibody to the beads using dimethy pimelimidate.
Please check link http://www.abcam.com/index.html?pageconfig...e&rid=10416
Hope this help.
Hi， Minnie Mouse， thank you! I think the method your recommended wil l be work. I'll try next days!