Bradford Protein Assay - (Feb/09/2001 )
The recommended wavelength for the Bradford Protein Assay is 595nm. I don't have accessto a microtitre plate reader with this filter at the moment. BioRad state that you can use a range of wavelengths between 465 and 595nm. Has anyone tried a wavelength other than 595nm?
Thanks in advance for any help.
My reader only allows me to read at 620!The proteins I have isolated seem to work.
You can make a wavelength scanning to see which wavelength you can use. The Biorad must have established their recommendation by this way.