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What is the best protein expression system - (Jan/15/2008 )

Hi
I need to clone and express a protein in E.coli, but do not know what expression system to use? there are many out there, but need an easy and quick one. Can anyone tell me please?

-MolecMicrobiol-

QUOTE (MolecMicrobiol @ Jan 15 2008, 02:38 PM)
Hi
I need to clone and express a protein in E.coli, but do not know what expression system to use? there are many out there, but need an easy and quick one. Can anyone tell me please?


There are a few systems out there, and the one you need really depends on what you want to do with the protein afterwards. I assume you want to purify the expressed protein, so consider a vector with an affinity purification tag, such as GST, MBP or hexahistidine. You might want to remove the tag after the affinity step, so use an online sequence checker (e.g. PeptideCutter) to check your protein isn't cleaved by the protease that is required to remove the tag. In my hands, I've had the most success with Promega vectors, but I'm sure others will work well too.

-gl24-