Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

protein samples on ice - how long is too long? (May/31/2006 )

hi all
just having a really paranoid moment - got fresh mouse intestinal polyps this morning and then promptly forgot them and left them in lysis buffer (as whole chunks) on (wet) ice for about 3 hours......will the protein still be ok for westerns??????


i think yes.
But best is to test.


I guess it shouldnt be a problem.


where is my post? I answered and it disappeared ! (may I need some rest?)

I will repeat my self
I don't think there will be a problem unless if you liberate the DNA. It will render the sample viscous, and all proteins will stick on the DNA.

If your sample is not viscous, then it's fine.