Protein extraction from solid matrix - (Apr/29/2005 )
I am trying to purify a protein from a PVDF or nitrocellulose membrane for MS/MS. Do you have a protocol to extract proteins from either membrane?
Although, I have never done the MS/MS procedure for my proteins, but I used to cut my band and send it to our MS/MS facility and got very good results. Therefore, you do not need to elute (extract) your protein from the membrane, you only need to locate your protein on the membrane and then cut it out as you do for SDS_PAGE gels and then send it for MS/MS, or perform tryptic digestion on that piece for MS/MS analysis. Good Luck!