vector for express secreted protein in vivo - (Aug/07/2006 )
Hi every one,
I want to over express a protein secreted into plasma of rodent. I think the Adeno virus is a good vector to express.
Could you please tell me where I can get an expression vector that have a leader sequence to secrete protein and a Tet-on regulation?
U will have to clone ur transgene into adenovirus vector. U could get it from some company or request from some labs doing the same.
There r gutless adenovirus with excellent expression of transgene. I think its thrid generation of adenovirus.
Remember for invivo studies, adenovirus has cytotoxic effect and also induced immune reaction.
how long do you want your protein expressed?
Thanks for your response, I just want its expression in short time at around 2 weeks