TCA radiacive protein precipitation without lysis - (Sep/17/2007 )
Hi,
I know from literature that it is posible to precipitate proteins with TCA from total cells- without lysing them before. But I cannot find any protocol for this procedure. Probally anyone can help me.
Up to know I have always lysed the cells in buffer with Triton X-100 or NP-40 before TCA precipitation. But now I need to precipirate radioactive proteins imiedetelly
-LUK-
QUOTE (LUK @ Sep 17 2007, 03:41 AM)
Hi,
I know from literature that it is posible to precipitate proteins with TCA from total cells- without lysing them before. But I cannot find any protocol for this procedure. Probally anyone can help me.
Up to know I have always lysed the cells in buffer with Triton X-100 or NP-40 before lysis. But now I need to precipirate radioactive proteins imiedetelly
I know from literature that it is posible to precipitate proteins with TCA from total cells- without lysing them before. But I cannot find any protocol for this procedure. Probally anyone can help me.
Up to know I have always lysed the cells in buffer with Triton X-100 or NP-40 before lysis. But now I need to precipirate radioactive proteins imiedetelly
it is a harsh method of lysis as proteins become precipitated, and you have to work with highly acidified lysates; use the same conc of TCA as for protein precipitation; work with cooling
-The Bearer-