how to precipitate the nucleicacid with out proteins - (Nov/09/2001 )
What form are you starting with. If it is tissue, then just get a nucleic acid isolation protocol from Maniatis or current protocols. If you seem to have protein contamination in nucleic acid samples, then do a phenol chloroform extraction followed by an overnight ethanol precipitation.
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I need a method for precipitate the nucelic acid without protien precipitation any one suggest me
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QUOTE (,Nov 9 2001, 11:00 PM)
What form are you starting with. If it is tissue, then just get a nucleic acid isolation protocol from Maniatis or current protocols. If you seem to have protein contamination in nucleic acid samples, then do a phenol chloroform extraction followed by an overnight ethanol precipitation.
could also try akaline lysis.............i think
-sarita4059-