brightest FACS fusion protein - (Dec/06/2007 )
I am trying to isolate a very small subset of cells within lung tumor- I am trying to design a mouse which expresses a fluorescent fusion protein driven by an endogenous promoter. These cells will be FACS sorted to isolate those which express the FP. I need to use an FP that is bright enough. Which FP is bright enough for FACS sorting of a small population?
What sorter do you have? Brightness is one thing, excitation is another. IF you have a (pretty standard) 488nm laser, you should be able to go with EGFP or Acgfp (EGFP is used the most I think). Check literature for toxicity of the fluorescent protein also!