8M urea buffer for protein extraction - (Oct/09/2008 )
I would like to prepare protein extranction buffer containing 8M urea.
Do I need to autoclave this solution before use?
-xksoyor-
No. 
-swanny-
No, you don't need to autoclave. But be aware that some protein quatification methods find very hard to measure protein concentrations in such a high urea concentrations...I had a coulple of troubles using 6M urea with 2 M thiourea for extraction buffer..my BCA anaylis went crazy.....all I could measure was urea....
good luck.
-Anuska76-
do not autoclave urea, it will break down.
if you need to sterilize a urea solution then do it by filter sterilization.
-mdfenko-