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RNA Related Discussions
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Can Taq Pol amplify template RNA? - (reply: 3)
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Protein isolation by Trizol - (reply: 1)
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RT-PCR for small RNAs - (reply: 1)
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To detect the DNA contamination in RNA - (reply: 2)
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Protocol for RNA preparation from Formalin Fixed tissues - (reply: 3)
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whole blood storage for RNA isolation - (reply: 3)
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Dot blot hybridisation of RNA - (reply: 2)
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RNA isolation from full thickness colon - RNA isolation, colon (reply: 2)
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Is incubation absolutely necessary for alcohol precipitation of RNA? - I'm doing HIV RNA isolation from plasma. (reply: 1)
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please! help on DNase treatment before microarray of RNA, which one?! - (reply: 4)
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Trizol in freezer - (reply: 2)
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RNA contamination in primers - (reply: 2)
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denaturing gel for RNA - (reply: 6)
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RNA gel - interesting bands? some help - (reply: 3)
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RNA gel - very interesting bands - (reply: 1)
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Can Nanodrop be used to check RNA integrity? - (reply: 2)
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RNA Electrophoresis Hints - (reply: 2)
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Trizol Reagent not Extracting RNA - was refrigerated, is this a problem? (reply: 1)
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please suggest me best rna isolation methods - available best methods and kits in market (reply: 4)
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RNA isolation by Trizol - (reply: 5)
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TRI Reagent Extration of small amounts of RNA - (reply: 5)
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RNA PCR - RNA PCR (reply: 4)
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RNA extraction from frozen cells suspended in PBS - (reply: 2)
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RNA clean up - RNA clean up (reply: 4)
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RNA sample precipitates when I add the 10X Buffer of the Turbo Dnase (AMBION) - RNA precipitation (reply: 1)
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RNA samples precipitates when I add 10X Buffer for Turbo Dnase treatment - RNA precipitation (reply: 2)
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RNA wash: 75% EtOH or 100% EtOH? - (reply: 1)
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Best storage temperature for samples - serum, protein, tissue, DNA, RNA... (reply: 5)
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heating RNA in water - (reply: 1)
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RNA, cDNA, PCR - RT Inconsistency - High Quality RNA, Working RT, Varying PCR Results (reply: 2)
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RNA Extaction from microglial cells culture - the best method for RNA extraction of microglial cells culture (reply: 2)
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QC in RNA extraction - Low A260/A230 ratio (reply: 2)
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Problems dissolving TriZol RNA pellet - (reply: 5)
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RNA conc importance? - (reply: 2)
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RNA extraction using qiagen RNeasy mini kit - (reply: 4)
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RNA conservation for Q-pcr and microarray - (reply: 2)
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Alternative RNA extraction - (reply: 1)
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RNAi of non-coding RNAs....? - (reply: 6)
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Rat DRGs - extracting RNA - Best method to prep for RNA extraction (reply: 2)
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mistakenly use RNeasy spin column while extracting RNA - (reply: 2)
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TRIzol wasn't homogenized with sample? - (reply: 4)
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RNA electrophoresis - different concentration of ethidium bromide between gel and buffer (reply: 2)
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HUVEC RNA isolation for microarray - What is best for microarray? (reply: 1)
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Protein come from nowhere - we cannot find RNA (reply: 5)
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RNA extraction for real time PCR from specific brain region - (reply: 1)
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low cDNA yield from bacterial total RNA. HELP! - (reply: 1)
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Decontaminate RNases from Solution - (reply: 2)
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Elimininating RNAses - (reply: 3)
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Need suggestions with working with RNA - (reply: 5)
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RNA isolation problem - (reply: 14)
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RNA storage at room temp? - (reply: 10)
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RT PCR of viral RNA segment - (reply: 1)
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RNA extraction - precipitation - (reply: 3)
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Difference between TBE- and Denaturing Agarose Gel Electrophoresis of RNA - Gel for RNA (reply: 2)
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How long can I store RNA at -20C? - (reply: 2)
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Electrophoresis RNA - (reply: 1)
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Cleaning up RNA after double enzymatic reaction - (reply: 3)
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Is there a way to extract RNA and protein from the same sample? - (reply: 2)
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Quality of RNA after extraction - (reply: 15)
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No cDNA from RNA sample - (reply: 4)
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About RNA extraction - Trizol (reply: 2)
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use of lysozyme in RNA extraction - (reply: 1)
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Three RNA bands after isolation - (reply: 2)
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TRIZOL for protein extraction - any problem? (reply: 4)
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where is the best place to perform the RNA extraction work and the relevant? - (reply: 2)
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harvest DNA or RNA from paraffinembedded tissue? - Is it possible to use a block of tissue for other analysis than IHC? (reply: 4)
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RNA gel - too many bands?? (reply: 3)
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Total RNA from mouse tissue - (reply: 3)
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When is the right time to adjust RNA concentrations for Rt-PCR? - (reply: 2)
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16s RNA of metagenomic DNA - soil bacteria (reply: 5)
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Stored RNA has precipitated - How to proceed? (reply: 1)
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RNA from paraffin - (reply: 1)
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Removing RNA from DNA during ChIP Assay - (reply: 3)
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How to converse RNA ug to mol? - (reply: 6)
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optimizing RNA isolation protocol - please help (reply: 2)
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Mycoplasma - How to detect and treat mycoplasma contamination in culture and RNA? (reply: 9)
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Microarray RNA extraction - (reply: 2)
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Does small amount of RNA affect MeDIP? - (reply: 3)
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how to convert ug to uL isolate of RNA? - (reply: 3)
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biotin-streptavidin hibridization - how can we detect biotin labeled RNAs with streptavidin HRP (reply: 2)
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Trizol extraction - (reply: 11)
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totalRNA isolation w/ Trizol for miRNA Array - (reply: 3)
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RPS18 = 18s rRNA ? - (reply: 2)
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Regarding RNA extraction - darn pellet (reply: 1)
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How much total RNA is needed to isolate and clone small RNA? - (reply: 1)
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RNA extraction from skin tissue - (reply: 2)
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RNA pellet can't dissolve in ethanol - (reply: 3)
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how to know RNA concentration? - (reply: 4)
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Primer extension and sequencing from total RNA - (reply: 1)
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RNA purification, am I going too far?! - (reply: 3)
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RNA isolation / Glycogen - (reply: 4)
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RNase A activity - (reply: 1)
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RNA isolation from Chicken liver - (reply: 3)
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Isoloation of total RNA and miRNA from blood ? - (reply: 1)
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RNA in expression array - (reply: 1)
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HELP- Can I save my RNA now? - RNAse free pipette tips (reply: 7)
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HELP- Can I get RNA out from samples now? - RNAse free pipette tips (reply: 1)
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FACS sorting into what solution for RNA isolation? - (reply: 2)
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optimize TRIzol protocol for RNA/DNA/Protein, HELP? - small amout of input material (reply: 1)
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Questions about PCR amplification using cDNA (RT from total RNA). - (reply: 6)
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RNA precipitation with DNase treatment - (reply: 1)
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RNA gel loading buffer? - (reply: 5)
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RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
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The best way for RNA extraction from cells in RNAlater? - TRI Reagent or RNeasy or something else? (reply: 1)
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Total RNA isolation for RT-PCR - (reply: 3)
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Isolating RNA from frozen tissues kept in vials - (reply: 4)
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problem in isolation of HCV RNA and then RT-PCR - RT-PCR, HCV virial RNA (reply: 1)
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Failed RT-PCR amplification of 3-&5''-ligated small RNAs - (reply: 5)
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RNAi effect-timing - (reply: 1)
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Aniline cleavage of RNA - (reply: 1)
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RNAi of two different genes - (reply: 4)
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How many ug total RNA needed in miRNA Northern blot - please help me (reply: 2)
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getting rid of genomic DNA in RNA extraction - (reply: 7)
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RNA isolation from small amount of suspension cells - (reply: 3)
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RNA extraction from human monocyte..why low yield? - (reply: 6)
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Can RNAi be used for silencing of non-coding RNAs? - (reply: 5)
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Resuspend RNA in TE or ddH2O? - (reply: 2)
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Help preparing TE to dissolve RNA - (reply: 2)
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Fishin out possible interacting protein for known RNA..?! - (reply: 1)
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New to RNA... Does this cDNA gel look OK? - (reply: 2)
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RNA extraction-PCI method-how to shear genomic DNA - method (reply: 2)
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looking for a 2-in-1 kit for Genomic DNA/RNA - (reply: 4)
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looking for Genomic DNA/RNA kit - (reply: 1)
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Combinatorial RNAi - double gene knockdown - (reply: 4)
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Getting RNA and Protein out of/off from Matrigel - (reply: 4)
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help please, RNA storage - (reply: 7)
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Ultimate RNA extraction from human pulp tooth - (reply: 2)
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small RNA target sequence - (reply: 1)
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Free trial kit for DNA, RNA, and protein extraction - AquaRNA - an aqueous DNA, RNA, protein extraction solution (reply: 4)
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How to clean the RNA buffer Tank (to get rid of RNase)? - (reply: 4)
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Intracellular bacteria RNA extraction - (reply: 1)
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Is it possible to isolate intact RNA in peripheral blood? - (reply: 2)
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RNA isolation from small amounts of cells - (reply: 1)
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RNA isolation from beads - (reply: 1)
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RNA denatruring agarose gel electrophoresis- help on sample preperation - (reply: 4)
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Question concerning quality RNA - (reply: 1)
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concentrating RNA - (reply: 9)
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Messy RNA extraction - Trizol protocol, odd pellet (reply: 5)
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calculating nunber of copies of synthetic RNA oligo - (reply: 1)
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how does rna know to use uracil and not thymidine - (reply: 3)
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RNAi side effects - (reply: 6)
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difficulty synthesising cDNA from total RNA - (reply: 26)
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Overdried pellets - RNA extraction - How to recover that jelly? (reply: 5)
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interaction of viral protein with RNAi machinery - (reply: 1)
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Grain RNA extraction - for barley (reply: 4)
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How much RNA at least on agarose gel? - (reply: 3)
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Intense background into wells when running RNA samples - (reply: 3)
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RNAi vector construction - Has anybody done it? (reply: 2)
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RNAi rescue by ectopic expression - (reply: 2)
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Easy-Blue for RNA extraction - (reply: 1)
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RNA extraction from plant cell cultures - (reply: 2)
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How much cDNA from RNA toata?! - (reply: 2)
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RNA degradation! - (reply: 3)
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RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
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Best method of storing cultured cells for later RNA isolation - (reply: 1)
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Hot Phenol and RNA shearing? - (reply: 3)
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Post sonication RNAse & Prot K treatment - What are the bands? (reply: 15)
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Removal of mitochondria RNA from total RNA? - (reply: 4)
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Need help in RNA extraction for soil - (reply: 2)
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amplificatin of bacterial total RNA - (reply: 1)
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RNAi - possibilities and applications - (reply: 3)
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3' RACE with just 10ng starting total RNA - (reply: 2)
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Question about TRIzol Extraction - (reply: 4)
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TaqMan - 18S curve not sigmoidal, replicates are different - (reply: 4)
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Suppression seen at protein levels but not at RNA - Pls Help - (reply: 5)
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Storing tissue for protein and RNA extraction? - (reply: 13)
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18S trouble in cleared MMG - (reply: 2)
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RNA extraction from seawater filters-welcome to the challenge - (reply: 2)
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RNA amplification - (reply: 3)
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Help-How is the RNA quality? - (reply: 3)
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Isolation of RNA from Difficult Tissues - (reply: 3)
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Trizol for RNA extraction - (reply: 4)
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why low spins recommended in RNA precipitation trizol protocol? - Gibco, later Invitrogen recommend only max 12000g - why? (reply: 2)
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RNA degradation after DNase1 treatment. - RNA degraded after DNase1 treatment. Any idea? (reply: 1)
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Ethanol precipitation- amount of RNA? - (reply: 1)
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need help with my rna gel - (reply: 12)
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Maintaining things RNase and DNase free - (reply: 2)
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RNA extraction from low amount of human tissue - Trizol-silica columns (reply: 1)
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Total RNA extraction from bacteria with Trizol : how much volume? - (reply: 5)
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trouble with DNase treatment of RNA sample - (reply: 3)
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how to remove contaminated RNA in DNA extract - (reply: 4)
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DNA and RNA concentration - (reply: 3)
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Difficulties using isopropanol in RNA extration - (reply: 5)
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RNA sample DNA ladder- how do they correspond? - (reply: 1)
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RNA extraction for ciliate protozoan - (reply: 6)
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Trizol stability - (reply: 7)
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RNA samples degraded in gel... where is the source of contamination? - (reply: 9)
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who has done biotin pull down RNA interacting protein before? - biotin pull down RNA interacting protein (reply: 3)
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RNA extraction from Bone Marrow - (reply: 4)
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RNA gel - multiple bands - (reply: 2)
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RNA Gels - (reply: 3)
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Control RNAi ! - (reply: 2)
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Question about RNA storage technique - many questions, please help (reply: 6)
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Help - RNA extraction from banana leaves - (reply: 2)
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RNA pellet - (reply: 6)
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In-situ Hybridization RNA probe - wrong size? (reply: 2)
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Help: Small RNA (siRNA) isolation from cultured cells - (reply: 5)
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western and trizol - western and trizol (reply: 1)
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Inheritance of RNAi - (reply: 4)
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Cleanign up RNA isolation - (reply: 3)
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RNA extraction from FROZEN blood - (reply: 7)
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from Trizol to phenol/chloroform for DNA extraction - (reply: 1)
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rna extracion problems - (reply: 1)
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RNA storage for microarray study - (reply: 1)
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How can I remove water from RNA? - (reply: 5)
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rna ratio - (reply: 4)
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Anyone going to Spain in May? - Interplay among genetics, epigenetics and non-coding RNA's (reply: 2)
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DNA, RNA, protein acetone precipitation? - (reply: 2)
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Nano Drop measurements and RNa Gels incompatible? - (reply: 2)
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Problem for DNA extraction (Trizol) - Problem for DNA extraction (Trizol) (reply: 2)
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Reading RNA ODs again.. - RNA extraction (reply: 6)
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Snap freezing tissue for RNA isolation - (reply: 10)
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Proteinase K treatment on viral RNA - (reply: 3)
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Good Review of RNAi - (reply: 2)
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Only 18S RNA Band shows up in RNA gel, why? - (reply: 2)
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RNA extraction - (reply: 9)
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RNA extraction - (reply: 2)
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Solutions for manual RNA purification - (reply: 2)
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Why should RNase treatment be followed by phenol chloroform - (reply: 4)
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Rnase protection assay queries - (reply: 1)
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RNA gel extraction - (reply: 2)
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RNA gel electrophoresis - multiple conformers or multiple products? - (reply: 1)
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how to get rid of mouse RNA - (reply: 1)
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ChIP: bright 300bp band after RNAse A - (reply: 1)
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Strange RNA behaviour - only 28S RNA left? - (reply: 2)
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Total RNA concentration - (reply: 1)
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RNA structure requirement for drosha-DGCR8 - (reply: 5)
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problems with RNA bands - on agarose gel (reply: 1)
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problems with RNA bands - on agarose gel (reply: 2)
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timing - DNA to RNA to Protein (reply: 1)
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RNA concentration issue for miRNA array - (reply: 4)
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RNA polymerase II antibody recommendation? - (reply: 2)
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RNA extraction from splenocytes - (reply: 2)
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RT-Pcr without RNA isolation? - (reply: 3)
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newbie questions on RNAi / microRNAs - (reply: 3)
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RNA extraction/electrophoresis problem - (reply: 8)
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reprobing small RNA blots with U6 - (reply: 1)
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Do you have protocol for isolation of RNA from seed? - (reply: 3)
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real time pcr with RNA co-precipitated with glycogen - (reply: 3)
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Does anyone have protocol for RNA extraction from root? - (reply: 2)
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can i see the RNA that isolate - (reply: 5)
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Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
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ask for RNAi vetor - (reply: 2)
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RNAi or Antisense oligos for inhibition in vitro and in vivo - (reply: 1)
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Protein extraction using trizol - (reply: 2)
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RNA extraction at high salinity - (reply: 1)
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How to sterilize mortar/pestle free of RNases - (reply: 5)
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Formaldehyde Gel Electrophoresis on non-denatured/denatured RNA - No/Faint RNA bands after denatration (reply: 1)
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how to get rid of the DNA within RNA extraction - (reply: 3)
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RNA liophylization - (reply: 5)
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Why my RNA absorbance reading shown negative? - (reply: 11)
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RNA extraction from flowers - (reply: 3)
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in vitro synthesis of RNA using T7 - (reply: 2)
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RNA Denaturation and Electrophoresis - RNA Bands Disappear after denaturation!!! (reply: 4)
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total RNA isolation from whole blood - (reply: 7)
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RNA extraction - How to take a pancreas sample and get RNA before degradation? (reply: 3)
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detailed function of na-acetate & chloroform:isoamylalcohol in RNA extractio - (reply: 1)
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RNA Isolation from hydatidiform mole - (reply: 2)
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Rnase Activity Assay - (reply: 5)
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Problem with RNA isolation/extraction of plasma - When RNA isolation form supernatant it's fine, not from plasma, us (reply: 1)
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ok to use Trizol w/ chloroform:isoamyl? - (reply: 2)
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Trizol contamination problem with Drosophila RNA Prep - (reply: 3)
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extractin RNA from cells grown on Matrigel - (reply: 1)
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How can I get rid of the DNA contamination when prepare my total RNA sampls for - I used the RNAqueous-4PCR (isolation of DNA free RNA kit)kit,still hav (reply: 8)
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non-specific binding in RNA in situ hybridization - (reply: 5)
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RNA Storage - (reply: 8)
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How do I know copies of RNA template added in a reaction ? - (reply: 4)
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RNA extraction from leukocytes - (reply: 2)
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RNA ratio in nanodrop - (reply: 6)
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RNA isolation from Groundnut seed - How to get rid off of oil content (reply: 2)
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Can RNase A degrade miRNA? - (reply: 12)
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After extracting total RNA from tissue using trizol reagent and phase lock gel,h - I am going to extract bothe total RNA and genome DNA from one sample (reply: 4)
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real time-RTqPCR RNA standard being difficult - (reply: 5)
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Help! DNA and RNA extraction - (reply: 3)
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RNA polymerase a sa fractionation control? - (reply: 3)
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Isolation of total RNA from different tissues - efficiency? - (reply: 2)
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RNA gel electrophoresis - any recommendations? - (reply: 12)
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Why do I got 3 bands of RNA gel? - (reply: 3)
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Aorta RNA isolation - (reply: 4)
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Total RNA isolation from mouse blastocysts - (reply: 1)
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RNAi and interferon induction - (reply: 6)
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troubleshooting in RNA extraction with Trizol - (reply: 3)
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RNA handling for RT-PCR? - (reply: 4)
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How can I send RNA samples for microarray application - general lab techniques (reply: 1)
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How to remove the DNase in a RNA or DNA sample? - (reply: 2)
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RNA extraction from alginate beads - (reply: 4)
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RNA Kit vs Trizol - (reply: 7)
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cy5 labelt RNA impossible to stain with sybrgreen II ? - (reply: 2)
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RNase decomtamination - (reply: 6)
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Problamatic A260/280 ratio of RNA - Problem with qualitative estimation of RNA (reply: 2)
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Loss of RNAi after changing culture media? - (reply: 2)
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isolation of total RNA from bacteria attached to HeLa cells - (reply: 1)
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RNA contamination? - (reply: 4)
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RNA purification from pancreas - troubles with the pancreas (reply: 1)
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Phenol-Chloroform at pH 8: Where's the RNA go? - (reply: 2)
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RNA extraction from plant tissue - all method are welcome (reply: 7)
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RNA contamination: how to get ride off it from extracted DNA? - (reply: 2)
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RNA extraction problem - (reply: 3)
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What is happening to my 18S? - (reply: 4)
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RNA in HeLa cell - amount of RNA in a HeLa cell (reply: 2)
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Autoclave reagents for rna? - (reply: 6)
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Extraction RNA (Tris-EDTA, DEPC-treated questions) - (reply: 6)
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any kit or reagent for extracting both DNA and RNA from the same cell culture - (reply: 3)
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RNA in wheat - (reply: 1)
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RNA extraction from pancreatic islets - (reply: 1)
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Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
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RNA extraction of bacteria - (reply: 3)
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contamination in RNA extraction - (reply: 4)
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Isolation of total RNA from whole blood - How much Trizol do I need? (reply: 3)
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genomic DNA extraction - more about Trizol (reply: 3)
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expression of short RNAs - (reply: 2)
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Does aza affect DNA/RNA quality? - (reply: 6)
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18S rRNA as internal control for mRNA-cDNA? - internal control (reply: 1)
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RNA isolation stationary phase - (reply: 1)
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How to wash pellet before RNA isolation - (reply: 1)
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RNA purification - A260:A230 ratio (reply: 2)
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RNA - (reply: 1)
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RNA quality for microarray - What must be the ratios? (reply: 4)
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RNA concentration and quality - How to be sure of RNA quality? (reply: 7)
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Help with Producing lentivirus in 293FT cells - Using Invitrogen's BLOCK-iT Lentiviral ol II miR RNAi Expression S (reply: 1)
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Methods for isolation RNA from blood without Kits - (reply: 1)
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Isolation of a specific mRNA? - RNA pull down (reply: 2)
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DNase treat my RNA prior to Generacer exp? - (reply: 1)
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Dissolving miRNA - What is the best way of dissolving RNA? (reply: 1)
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RNA degradation - (reply: 8)
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RNA extraction from eosinophils - (reply: 3)
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E.coli Total RNA's size - Any literature? (reply: 2)
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How to test whether total RNA is changed or not? - (reply: 3)
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Blood RNA extraction - Can the Qiagen RNeasy mini kit extract RNA from blood? (reply: 3)
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total RNA isolation - total RNA isolation (reply: 5)
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Reverse-transcription - how much RNA will be converted into cDNA (reply: 4)
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isolation of small RNAs from culture supernatant - (reply: 11)
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total RNA analysis - (reply: 1)
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RNA stuck in the wells - (reply: 1)
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RNA Isolation - Fragmented RNA (reply: 1)
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what is vault RNA ? - (reply: 2)
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Rat Brain Tissue RNA Isolation with TRIzol - (reply: 9)
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RNase treatment - genomic DNA sample lost during purification - RNase treatment troubleshooting (reply: 1)
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Total RNA extraction using TRizol for isolating miRNA - (reply: 6)
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how to recognise the diferent units of rDNA? more easily RNA structure drawing - help! (reply: 2)
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Protocols for RNA extraction from 5-10mg starting tissue samples - Molecular Biology (reply: 1)
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18s control higher then suppose to be - (reply: 2)
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RNA extraction - didnt get ratio 2:1 ( 28s rRNA : 18 s rRNA) - RNA extraction from Tilapia (reply: 2)
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Problems with RNA extraction or QPCR for AI - (reply: 2)
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RNA extraction from parasite - (reply: 2)
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RNA isolation from mouse heart tissue - (reply: 2)
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wht is the best way to extract RNA from oocytes stages - (reply: 1)
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sodium acetate - the function of soduim acetate in precipitation of DNA/RNA (reply: 2)
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reverse transcription from viral rna - (reply: 9)
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RNA quantification for Real Time RT-PCR - (reply: 2)
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RT-PCR without RNA isolation - (reply: 2)
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cell line rna extraction - (reply: 1)
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Does non-digested RNA in cDNA sample have an effect on RT-PCR quantification? - (reply: 2)
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RNA expression calculations - (reply: 1)
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DNA, RNA storage - (reply: 6)
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RNA re-extraction with phenol - I can't get a good RNA quality (reply: 3)
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RNA stain on blot? - is there a way to see the total RNA on a blot? (reply: 1)
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minimum amount of rna in RT-PCR - (reply: 1)
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RNAi targeting one gene in a bicistronic transcript - Will the other gene be knock down? (reply: 4)
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EGTA vs EDTA and high phenol PH for RNA isolation from small amount of root tips - (reply: 1)
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A 270 for RNA - (reply: 1)
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avicel medium for RNA extraction - crushing of avicel (reply: 1)
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low OD260/280 ratio for RNA extraction with TRIzol - (reply: 5)
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RNA isolation from frozen blood - RNA isolation (reply: 3)
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RNA extraction from oocytes. - (reply: 3)
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RNA Dotblot issues - Alkaline or formamide/formaldehyde desnaturation? Control (reply: 7)
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Detect small RNA using DIG-labeled probe - Northern for small RNA (reply: 3)
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ask for help about the RT-PCR - does different aoumnt of RNA affect the RT efficiency? (reply: 1)
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RNA isolation from blood - (reply: 1)
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on-collumn DNASe in RNA purification - (reply: 3)
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RNA transfection VS Plasmid DNA transfection - (reply: 3)
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18S Melt curve - sorry for the cross post.. this forum gets more traffic. (reply: 1)
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will purification processes lead to loss of RNA? - (reply: 1)
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How pure are my RNA samples - (reply: 6)
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chronic exposure to Trizol (phenol)? - (reply: 4)
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qPCR vs RNA protection assay - (reply: 1)
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RNA quality from total blood - (reply: 6)
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RNA extraction from TissueTek embedded tissue - (reply: 1)
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condition for keeping integrity of RNA for RT-PCR - (reply: 1)
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RNase monster - how to avoid RNA degradation? - (reply: 4)
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beginners guide to stable RNAi transfection - (reply: 1)
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RNA AMOUNT FROM LOW NUMBER OF PBMC - (reply: 2)
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Is my plasmid contaminated by RNA? - (reply: 13)
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What's the difference between using DNA and RNA polymerase for PCR - (reply: 2)
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RNA extraction - (reply: 15)
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Is my RNA dead? - freezing cells after adding RNA lysis buffer (reply: 2)
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RNAse and DNA digestion in Southern Blot - (reply: 3)
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RNase and DNase free? - water (reply: 2)
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Problem with agarose gel? or with RNA ? - (reply: 7)
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determining efficiency of RNase treatment - (reply: 1)
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RNA extraction of a very, very small tissue sample - (reply: 2)
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RNA extraction methods for callus - (reply: 1)
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FTA card for Total RNA Extraction - does anyone have experience??? (reply: 1)
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Viral RNA isolation from plant - (reply: 4)
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some formulas - convert ng DNA/RNA/ml into copies/ml (reply: 3)
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RNA in cDNA - Can I use the same PCR primers for the production of cDNA? (reply: 3)
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Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
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Any kit out there for protein and RNA isolation from monocytes? - (reply: 4)
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RNA extraction from mouse splenocytes in 96 well plate - (reply: 1)
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total rna isolation from cockroach - (reply: 2)
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How long can extacted total RNA last? - (reply: 11)
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RNAse Protection Assay for microRNA - is anyone using the mirVana Detection Kit? (reply: 2)
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Co-Purification of RNA and DNA - Has anybody any experience with the new Allprep Kit by Qiagen? (reply: 7)
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Restriction enzyme specificity for thymine and DNA - are thymine/uracil and DNA/RNA interchangeable? (reply: 1)
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RNA concentraion - (reply: 2)
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DNase treatment of RNA prior to RT-PCR - (reply: 4)
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How to remove gDNA from small amounts of RNA - (reply: 4)
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DNA free RNA for PCR - (reply: 6)
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DNase treatment of RNA - (reply: 2)
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RT-PCR on 3kb fragment of viral RNA - (reply: 2)
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problem with RNA isolation from leaves - degradated (reply: 2)
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Silly question, fidelity of RNA polymerase - transcription is 100% error free ? (reply: 11)
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Smear on gel after MiniPrep - Is this RNA? (reply: 10)
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Which primer concentration for RT with very low RNA amounts? - (reply: 1)
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RNA extraction/troubleshooting - specifically for older tissues (reply: 2)
-
Poor RNA quality - (reply: 9)
-
RNA migration in agarose formaldehyde gel - (reply: 6)
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RNA contamination in cDNA for microarray experiments - RNA contamination in cDNA for microarray experiments (reply: 3)
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How to concentrate RNA - (reply: 2)
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DNA isolation: RNase A or B - which use? - (reply: 3)
-
difference between RNA & cDNA gel image - (reply: 1)
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DNA/RNA isolation from small starting material? - Which is the best method RNeasy, Trizol, etc? (reply: 2)
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RNA quality - (reply: 10)
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Is RNAi patented? - (reply: 4)
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preservation of tissues for later on RNA isolation. - (reply: 3)
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defroze RNA for too long - (reply: 2)
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Time taken for RNA to denature - (reply: 2)
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isolating RNA from FACS - (reply: 2)
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RNA Extraction Method - (reply: 2)
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RNA isolation from Oocytes - (reply: 4)
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How to isolate RNA from zebrafish tissue? - (reply: 1)
-
Why does changinf buffer effect isolation of RNA? - (reply: 1)
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Why is EB added into RNA loading buffer? - (reply: 4)
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Trizol protein extraction - (reply: 2)
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RNA won't dissolve in DEPC water - (reply: 4)
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RNA gels - good protocol required (reply: 2)
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RNA extraction from formaldehide fixed cells - (reply: 2)
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T4 DNA ligase and RNA - (reply: 2)
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18S as hk, how can i optimise it? - (reply: 1)
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concentrate RNA - (reply: 3)
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RNA at 50 degree overnite, degraded? - (reply: 3)
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how to measure DNA quantity, exclusive of RNA - (reply: 4)
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DNase treatment in RNA isolation - protocol for DNase treatment (reply: 4)
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RNA gel shift - (reply: 1)
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Is all lab bacterial strains have t7 RNA polymerase gene... - (reply: 4)
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total RNA from bone marrow - (reply: 1)
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Which method of RNA staining is less costly - (reply: 3)
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Small fragment RNA isolation from interacting protein - (reply: 1)
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problem with RNA extraction using TRIZOL - (reply: 2)
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Time point for reduction of protein levels after RNAi - (reply: 1)
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quanitation of protein extracted with trizol - (reply: 7)
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RNAi doesn't work? - (reply: 5)
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RT-PCR problems on mouse ear RNA - (reply: 1)
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How much RNA is needed for labelling and how much labelled cDNA is needed for hy - (reply: 4)
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Rnase A amount needed - (reply: 1)
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cannot see RNA on gel - (reply: 6)
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Protocol for RNAse treatment of genomic DNA - pre-RT-PCR (reply: 3)
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RNA dot blot probe not detecting mRNA - (reply: 1)
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How to do Per cell RNA concentration? - (reply: 2)
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what happens when a cell gets frozen without cryopreservative - can polysomes and RNA be extracted from such cells? (reply: 4)
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rna isolation from bone marrow cells - (reply: 1)
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Total RNA from archival tissue that is fixed and paraffin embedded (like brain t - (reply: 1)
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RNA extraction and purification - (reply: 6)
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How to identify an unknown RNA? - (reply: 2)
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what's the sufficient concentration of RNA for reverse transcription? - (reply: 7)
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Total RNA extraction from ethanol preserved samples? - (reply: 7)
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Can you concentrate RNA in Plasma? - (reply: 4)
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Best methods for storing RNA - (reply: 7)
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Why do we have to use MOPS and formaldehyde in RNA electrophoresis - (reply: 1)
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the way of viral RNA isolation from serum - (reply: 4)
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What does "S' mean in 18S or 28S RNA? - (reply: 2)
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Is a rat being frozen under -20 for two days still good for RNA isolation? - (reply: 4)
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Handling small quantities of RNA - tips? - spec quantification of ~50ng? (reply: 5)
-
siRNA - sh and si RNA RJNAi (reply: 6)
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RNA isolation - RNA isolation (reply: 3)
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EtOH precipitation of RNA problems - (reply: 5)
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total RNA isolation question - (reply: 8)
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How to treat total bacterial cell DNA with RNase - I need to get RNA free DNA (reply: 6)
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How to remove traces of ethanol after RNA wash - (reply: 5)
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Problem with DNase digestion of RNA - (reply: 6)
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How can I extract intact total RNA from frozen HUman Skin? - This cumbersome work really kills me! (reply: 5)
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Rna isolation from cyanobacteria - (reply: 1)
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How to eliminate DNA contaminaiton during RNA extraction? - (reply: 5)
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Off-target effects of RNAi - (reply: 4)
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How to increase RNAi efficiency by siRNA transfection? - (reply: 4)
-
Isolation of RNA from platelets - help! - RNA isolation platelets (reply: 1)
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Native RNA gel - (reply: 1)
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Need protocol for RNA isolation from PBMC - (reply: 2)
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My Total RNA degraded? - (reply: 3)
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RNA activation but not RNAi? - (reply: 8)
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RNA isolation from locus ceruleus - (reply: 2)
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Why using acid guanidium-phenol-chloroform on RNA extraction - (reply: 3)
-
isolate RNA from small size sample - (reply: 1)
-
ELISA and RNA purification - Can ELISA be performed on frozen leafs (reply: 4)
-
RNA extraction after H&E - (reply: 1)
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RNA isolation from trypsinized cells - (reply: 4)
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DNA:RNA hybrid - is it more stable than DNA:DNA or RNA:RNA (reply: 4)
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RNase treatment - (reply: 9)
-
How to make Tris RNAse Free? - (reply: 5)
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RNA extraction from whole blood using TRIzol - (reply: 1)
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ASK FOR HELP: why I can not get total RNA from human liver - (reply: 5)
-
RNAi of HepG2 cells - low transfection effeciency (reply: 1)
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problem in RNA extraction from mice liver and spleen - (reply: 2)
-
bleach pretreatment for RNA extraction - (reply: 1)
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RNA extraction from muscle tissue using RNeasy kit - Can I merge these two protocols together? (reply: 2)
-
RNA Amplification - (reply: 2)
-
Stratagene Reference RNA - (reply: 1)
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RNA extraction - nuclear and cytoplasmic fractions - (reply: 3)
-
Small amounts of RNA for qRT-PCR - (reply: 7)
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RNA isolation from Fungus - (reply: 1)
-
RNA polymerase? - (reply: 5)
-
I don't know if I have RNA pellet or not - There are very little amount of RNA, Is there anything I can see to see the pell (reply: 3)
-
Increased transcripts after RNAi - (reply: 10)
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Ratio of phenol:chloroform in RNA extraction - 1:1 or 5:1? (reply: 2)
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Help! DNA contamination... How to get rid of it? - Trizol RNA isolation (reply: 3)
-
Northern is smeary but you can see 28S and 18S bands - (reply: 1)
-
RNA extraction from cells grown in matrigel - (reply: 1)
-
Series dilution of RNA or cDNA for qPCR standard curve? - (reply: 1)
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why transfected cells has less RNA? - (reply: 2)
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Is DNAse treatment essential during RNA extraction - (reply: 1)
-
small RNA (100-500bp) - (reply: 1)
-
Strange thing about RNA concentration and real-time melting curve - (reply: 4)
-
Is there any method out there to quantify small amount of RNA (around 1-2ng/ul)? - (reply: 2)
-
nucleacid and protein - Protein, DNA and RNA (reply: 2)
-
Problems with protein extraction with Trizol - (reply: 1)
-
RNA ectraction from whole blood using TRIzol LS - (reply: 6)
-
RNA Oligos VS DNA oligos - Dumb Question but need a straight answer (reply: 4)
-
faster way to identify RNA virus other than RT-PCR? - (reply: 2)
-
total RNA extraction from infected protoplasts - (reply: 7)
-
cloudy RNA preparation - (reply: 2)
-
RNA and poly(a) RNA extraction problems - (reply: 10)
-
formaldehyde in RNA gels - (reply: 4)
-
Rna semi dry transfert overnight... - is it possible? (reply: 3)
-
RNA gel troubleshooting - (reply: 8)
-
RNA half life - (reply: 16)
-
best kit for RNA extraction from animal tissue - (reply: 3)
-
gDNA and RNA extractions from whole blood - (reply: 1)
-
HELP! muscle RNA extraction - (reply: 3)
-
RNA isolation problems - Have a look at my spectrum... (reply: 12)
-
RNA 260/230 - really not THAT important? - (reply: 6)
-
RNA extraction - (reply: 1)
-
Trireagent or Trizol isolation - (reply: 1)
-
Urea PAGE for RNA....why? - (reply: 3)
-
RNA labeled at the 3'-end - (reply: 1)
-
RNA precipitation - (reply: 5)
-
RNA extraction from small cell number - (reply: 4)
-
RT-PCR mouse RNA released from complement-lysed cells - Goal: to whether RNA is of nuclear or mitochondrial origin. Planning to PCR ampl (reply: 2)
-
miRNA extraction using Trizol - (reply: 10)
-
RNA extraction without using liquid nitrogen - Can it be done? (reply: 3)
-
Size of 23s and 16s RNA different - (reply: 3)
-
Eukaryote's RNA Polimerases - transcription 3' to 5'? (reply: 1)
-
Denaturing Agarose gel for RNA - (reply: 10)
-
BRAIN RNA EXTRACTION! - (reply: 3)
-
Different RNA sources, Different PCR optimal condition? - (reply: 5)
-
protein precipitation / RNA binding factors - (reply: 3)
-
Multiple bands on RNA gel, why? - (reply: 6)
-
Interphase problems with TRIzol RNA extraction - And a question about reprecipitating RNA (reply: 5)
-
RNA isolation from frozen tissue sections - Which Kit do YOU use? (reply: 3)
-
Problem ratio with RNA extract - (reply: 4)
-
Has anyone seen target mRNA increasing after RNAi? - (reply: 7)
-
detergent in RNA extraction - (reply: 3)
-
RNA from plant tissue shows 3 rRNA bands - RNA isolation by Trizol method (reply: 2)
-
problems during RNA brain extraction - (reply: 1)
-
DNA / RNA extraction from freeze dried tissue - (reply: 6)
-
RNA binding protein isolation - how to figure out what binds your mRNA? (reply: 1)
-
Protocol for RNase treatment of plasmid for RNA cleanup - (reply: 4)
-
Small RNA extraction - iRNA (reply: 12)
-
RNA dot blot - is the denaturation step important? (reply: 5)
-
help for total RNA from skin mice - (reply: 2)
-
RNA labelling for Microarrays - (reply: 5)
-
RNA extraction from bone - (reply: 1)
-
RNA spectrophotometry - (reply: 7)
-
RNA isolation from castor leaf sample using GTC method - unable to detect RNA bands in castor leaf sample using GTC method (reply: 1)
-
Position of RNAi target on mRNA - How important is the location (reply: 2)
-
Northern blot protocol for small RNAs by PAGE - (reply: 1)
-
RNAi confusion - More expression! (reply: 7)
-
RNAi in yeast? - (reply: 3)
-
Double-stranded RNA to RNA ligation - (reply: 1)
-
extraction RNA from coronary artery endothelial cells - yield problem (reply: 1)
-
gels for RNA - (reply: 1)
-
RNA extraction - freezing homogenate - (reply: 1)
-
RNA extraction - (reply: 4)
-
RNA stability and Long term storage of homogenate in Trizol or similar reagent - (reply: 2)
-
Minimum amount of RNA for real-time PCR - (reply: 4)
-
RT-PCR question - genomic DNA amplified with RNA (reply: 9)
-
DNAse treatment in same step of RNA extraction - (reply: 5)
-
brain RNA isolation - problem RNA integrity (reply: 3)
-
ribosomal RNA contamination - (reply: 3)
-
RNA extraction from small number of cells - (reply: 3)
-
How to design siRNAs for RNAi - (reply: 2)
-
DNase I Amplification grade and DNase I for RNA treatment - (reply: 1)
-
How reliable is 18S RNA as reference - (reply: 4)
-
isolation of RNA from samples stored in RNA later (ambion) - can we use Qiagen total RNA isolation kits (reply: 3)
-
RNA pellets seem too big: normal or something else in there? - (reply: 6)
-
Losing my RNA pellets ! - (reply: 8)
-
RNA Isolation from Breat Normal tissue - (reply: 3)
-
A question on RNAi efficiency? - (reply: 5)
-
RNA extraction from bull sperm - (reply: 5)
-
RNA EXTRACTION - (reply: 4)
-
RNA integrity - (reply: 4)
-
problem with RNA ladder in polyacrylamide gel - (reply: 1)
-
RNA extraction - chloroform - (reply: 3)
-
Problem extracting RNA using Trizol - (reply: 12)
-
how to start for effective RNAi screening--scale up question - (reply: 2)
-
RNA extraction - (reply: 1)
-
rebelK RNA - (reply: 1)
-
Cheap RNA isolation method instead of TriZol - (reply: 3)
-
RNA storing/RT PCR using oligo dT - (reply: 5)
-
cloning/amplifying attenuate RNA transcripts - (reply: 6)
-
RNA degredation - Extracting RNA from Brain Tissue (reply: 8)
-
Viral RNA detection - (reply: 1)
-
Inconsistent results with RNA quantification at A260/280 - AND problems with recoveries using Qiagen columns (reply: 7)
-
Phenol purification of genomic DNA degraded by RNA? - (reply: 4)
-
Starting real time rt-pcr - working with rna virus (reply: 7)
-
How Ethidium Bromide intercalates between RNA? - (reply: 9)
-
Isolate tRNA from total RNA - (reply: 2)
-
quantity of RNA to use in RT reaction - (reply: 3)
-
Agarose gel - DEPC-induced fragility? - RNA gels (reply: 2)
-
especial cell line and gene, what is the result of RNA? - select a more inexpensive siRNA (reply: 2)
-
Is it necessary to treat total RNA with DNAse I when isolating mRNA? - (reply: 16)
-
Problem in RNA isolation, degraded, even though I pay extra attention to prevent - (reply: 14)
-
Cellular RNA extraction - Sequence-selectivity - (reply: 2)
-
co-transfecting DNA and RNA duplexes - (reply: 3)
-
Easy protocol for purification of in vitro transcribed RNA oligonucleotides? - (reply: 2)
-
RNase and RNase A? - (reply: 4)
-
RNA pol I, II and III - biochemical differences? - (reply: 1)
-
RNA-gel; how much RNA can I use? - (reply: 3)
-
Question about doing RNAi in primary cells - (reply: 3)
-
Knockdown protein expression using rnai - (reply: 4)
-
Isolating RNA from a small number of cells - (reply: 1)
-
RNA isolation from tissues: RNA pellet is yellow-brown? - Why this? (reply: 2)
-
RNA isolation: ethonal wash step - Do you resuspend the RNA pellet by pipetting? (reply: 4)
-
How do YOU precipitate your RNA? - (reply: 2)
-
How to accurately quantify plasmid DNA in solution within impurity of RNA? - (reply: 3)
-
help with buffer 5.1 for RNA extraction - (reply: 6)
-
micro RNA traget prediction - (reply: 5)
-
RNA contamination w/ ethanol - (reply: 18)
-
RNA extraction from formalin fixed paraffin embedded tissue - protocol? (reply: 1)
-
Trizol vs TriReagent for RNA extraction - (reply: 3)
-
more RNA storage questions - (reply: 12)
-
RNA prep (Trizol) with small cell numbers - How low can you go? (reply: 13)
-
RNA extraction after magnetic beads positive selection - Best kit / procedure??? (reply: 1)
-
what is the difference about siRAN, miRNA, shRNA and RNAi - (reply: 1)
-
Strange nanodrop readings after RNA isolation, strange solution - (reply: 13)
-
DNA ladder for RNA analysis? - (reply: 2)
-
RNA pol II driven shRNA expression - CMV driven shRNA expression (reply: 10)
-
High throughput RNA extraction - (reply: 9)
-
RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
-
RNA Isolation using Trizol - Getting an additional band and v.low 260/230 (reply: 2)
-
RNA extraction from blood - (reply: 9)
-
RNA Quantitation - Quantitation in presence of Glycogen (reply: 1)
-
How to characterize unknown small RNA - (reply: 3)
-
DNAse I treatment of RNA - (reply: 2)
-
RNase treatment of plasmid - (reply: 7)
-
RNAi in a sense/antisense transcript system - (reply: 3)
-
RNA Isolation with RNeasy - (reply: 6)
-
Trizol RNA isolation - (reply: 1)
-
Help RNA and protein extraction - i dont understand the chemistry behind it (reply: 3)
-
DNA, RNA and protein extraction using basic solution - (reply: 4)
-
Sonication & RNA - (reply: 1)
-
DTT & RNase inhibitor in RT reaction - (reply: 6)
-
RNA extraction from fungi for microarrays - advice is needed (reply: 1)
-
RNA preparation for mycobacteria - (reply: 7)
-
Help me ! I want to do RNAi - (reply: 1)
-
DNA extraction by TRIzol - (reply: 2)
-
Protocol for DNase I treatment of RNA - (reply: 4)
-
RNA sample stuck in well during agarose electrophoresis - (reply: 1)
-
Problems with Trizol - (reply: 15)
-
Does RNA degradate in the refridgerator (4C)? - (reply: 5)
-
Spce RNA/DNA Quartz curvette? - (reply: 4)
-
RNAse free water - Manufacturer (reply: 6)
-
keeping total RNA input costant - (reply: 6)
-
RNA extraction from sludge - (reply: 2)
-
16sRNA, bacteria Identification - (reply: 1)
-
16sRNA, identification of Bacteria - need the protocol (reply: 1)
-
total RNA isolation from matrigel coated plate - (reply: 5)
-
RNA extraction - (reply: 1)
-
total RNA isolation - (reply: 6)
-
total RNA isolatin - (reply: 2)
-
Regulation of protein may not be detected at RNA level - Can anyone tell me why??? (reply: 3)
-
RNAi negative controls - (reply: 2)
-
RNA, cDNA, and DNA - RNA, cDNA, and DNA (reply: 3)
-
RNA extraction with smear after electrophoresis - (reply: 1)
-
genomic DNA extraction for PCR - Can't get 16Sr RNA.... (reply: 1)
-
RNA extraction by Trizol protocol - I didn't get a good quality of RNA (reply: 1)
-
total RNA isolatin - (reply: 3)
-
blood RNA and DNA isolation problems? - (reply: 1)
-
A few questions on RNA extraction and stability - (reply: 12)
-
Checking integrity of total RNA using gel? - (reply: 6)
-
RNA extraction kit - What do you think about this one? (reply: 2)
-
RNA Gel Problems - RNA not showing up as bands in gel (reply: 6)
-
phenol:chloroform:isoamyl alcohol at different pH for RNA extraction - (reply: 1)
-
WBC RNA preservation and purification (NEED HELP!) - (reply: 3)
-
RNA storage - share your experience how you store RNA - (reply: 20)
-
Plant leaf RNA extraction using Trizol reagent - (reply: 1)
-
does phenol extraction denature double stranded DNA or RNA? - (reply: 1)
-
human RNA contaminated with bacterial RNA - give your opinion about a RNA gel (reply: 8)
-
rat 18s rRNA primer - designing primers (reply: 1)
-
Does RNase keep activity in the 50%DMSO - (reply: 1)
-
Percentage of SDS to denature RNases? - (reply: 3)
-
Can 0.22 µm filters get rid of RNases? - (reply: 8)
-
Northern Hybridization - qestion about RNA degradation - need some clues (reply: 2)
-
RNA in situ hybridization - Whole mount RNA in situ hybridization on vertebrate embryos (reply: 2)
-
RNA preparation from in vitro transcription - (reply: 6)
-
Upcoming conferences? - RNAi conferences (reply: 3)
-
Does anyone have experience to extract RNA from frozen blood? - (reply: 7)
-
total RNA from BSE infected WBC for microarray and qPCR - (reply: 2)
-
DNA & RNA Isolation - can any person give me protocol for this (reply: 4)
-
RNA gel problems - (reply: 2)
-
How to choose the best optimum RNAi kit? - (reply: 1)
-
DNase I treated RNA... - (reply: 1)
-
RNA electrophoresis- samples smearing but NOT degraded - (reply: 3)
-
Designing RNAi construct for adult Drosophila - (reply: 1)
-
Viral RNA extraction - Formalin fixed & Paraffin embended tissue (reply: 17)
-
RNA Formaldehyde Gel problem - (reply: 5)
-
A1/A2 RNA ratios: troubleshooting - what does a ratio greater than 2.0 indicate? (reply: 3)
-
RNA extraction with TRizol and RT-PCR - (reply: 8)
-
Difference between DNA and RNA extraction with phenol - DNA or RNA extraction with phenol (reply: 7)
-
RNAi using SIN-lentivectors BSL 2 plus? - (reply: 1)
-
in situ hybridization and RNA probe design - (reply: 6)
-
Help needed for removing RNA in DNA extract. - (reply: 8)
-
Is there a program for finding secondary strutural RNA elements? - finding secondary strutural RNA elements (reply: 2)
-
Is there article about lymphoma therapy with RNAi? - (reply: 1)
-
RNA Isolation - Increasing RNA amounts quantitatively and qualit. (reply: 3)
-
RNA quality problem - (reply: 4)
-
poor RNA quality - (reply: 5)
-
large RNA northern - (reply: 2)
-
Help! RNA in vitro transcription - (reply: 3)
-
DNase I treatment of RNA sample - (reply: 10)
-
18S rRNA copy number - (reply: 1)
-
Start with a potential non-coding RNA sequence - (reply: 3)
-
Automated DNA/RNA extraction, would you use it? - (reply: 1)
-
About RNAi based on sh-RNA plasmid - (reply: 2)
-
Concentrating RNA advice needed - (reply: 1)
-
RNA storage - -20 or-70°C? (reply: 2)
-
Phenol+pH for RNA extraction - phenol-chloroform isoamylalcohol extraction (reply: 4)
-
RNase in RNA - QC for RNase in RNA (reply: 3)
-
RNAlater and RNA extraction - Crystal formation with RNALater (reply: 3)
-
HelP!some problems during the RNA extraction! - (reply: 2)
-
Can't get rid of RNA in my phage extraction. - (reply: 4)
-
DNA + RNA extraction - (reply: 1)
-
RNA extraction from nitrogen-frozen tissue - (reply: 2)
-
Extracting RNA from skin - Isolation and concentration (reply: 1)
-
DNA and RNA Extraction - Help (reply: 9)
-
questions about RNase A storage - enzyme manipulation (reply: 2)
-
Trizol treatment - (reply: 7)
-
Tough Bacteria Break Up Method? - Isolation DNA and RNA from Gram Positive Staphy (reply: 3)
-
inactivating DNA/RNA with 70% ethanol - (reply: 3)
-
Saliva RNA Preservation - Complete deactivation/inhibition of saliva RNAses? (reply: 2)
-
RNA isolation - question about kits (reply: 3)
-
RNA extraction from liver - (reply: 4)
-
RNA isolation from bacteria - kit recommendation (reply: 2)
-
DNase I treatment of RNA dissolved in TE buffer - (reply: 2)
-
RNA electrophoresis problem - RNA not denatured (reply: 9)
-
RNA degrade - RNA degrade during in DNase treatment (reply: 4)
-
How do you store your RNA? - (reply: 2)
-
RNA gel - 28S band (reply: 2)
-
RNAi knockdown versus DNA knock-out - (reply: 2)
-
RNA Spectrophotometry Troubleshooting - (reply: 3)
-
RNase treatment - (reply: 3)
-
what is a good kit to isolate cellular RNA from human blood? - (reply: 1)
-
Housekeeping Gene Selection / RNA purification troubles - Molecular Biology (reply: 2)
-
RNA later - (reply: 6)
-
RNA purification from macrophages - (reply: 5)
-
how much total RNA do you use for RT-PCR - (reply: 6)
-
qiagen column recycling - cleaning column of qiaamp viral rna mini kit (reply: 4)
-
RNA isolation - DNA contamination troubles (reply: 6)
-
vortex RNA? - (reply: 1)
-
RNA in vitro transcription - kits, protocols - recommendations? (reply: 6)
-
Software for transformating RNA into cDNA - (reply: 1)
-
RNA isolation - i want to show a gel (reply: 4)
-
RNA precipitation - How long should I precipitate my RNA? (reply: 2)
-
DNA/RNA isolation from same sample simultaniously - help me (reply: 1)
-
DNA/RNA ratio - techniques (reply: 1)
-
Total RNA from lymphocytes - Problems with RNA yield (reply: 2)
-
Internal Control RNA & RT-PCR - (reply: 1)
-
DNA contamination in RNA extracted with RNeasy kit - (reply: 3)
-
extract RNA from frozen cells - (reply: 6)
-
Which buffers for storing of RNA and DNA? - (reply: 6)
-
RNA stability in long term storage - (reply: 2)
-
RNA isolation - combination of Trizol & colum-based kit (reply: 6)
-
RNAi in CHO cells - (reply: 5)
-
What is the BEST way to extract total RNA? - (reply: 12)
-
Quality of RNA by spectrophotometer - (reply: 3)
-
No change in mRNA or protein levels after RNAi in Trypanosoma brucei - (reply: 4)
-
RNA vs DNA purifucation - how are they separated from each other? (reply: 5)
-
anyone using TRIzol from invitrogen? - (reply: 3)
-
"cDNA must accurately represent RNA input amounts" - (reply: 1)
-
reverse-transcribed RNA - (reply: 1)
-
Phenol:chloroform RNA extraction method - How it work... (reply: 2)
-
transport RNA and cDNA - (reply: 2)
-
Morphologic changes of HeLa cell after RNAi - (reply: 7)
-
RNA Quantification by spectrometer - 260 nm vs 230 nm (reply: 2)
-
DNA contamination in RNA extraction - (reply: 2)
-
Trizol followed by column purification - (reply: 9)
-
Does DMSO destroy RNase?? - (reply: 2)
-
Tissue storage for RNA isolation - (reply: 4)
-
DNase treatment for removing DNA from RNA samples - (reply: 1)
-
RNA isolation with TRizol - basic Q (reply: 3)
-
Do I have amplication of RNA by qPCR? - (reply: 7)
-
Do I have amplication of RNA? - (reply: 1)
-
Removal of RNAse from pipette tips??? - (reply: 8)
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Degraded total RNA - (reply: 6)
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Good protocol for DNase treatment of total RNA - (reply: 1)
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DNA "clean-up" - How to get rid of DNA contaminants: RNA, proteins (reply: 1)
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RNA storage and shippment - (reply: 8)
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Quality RNA - (reply: 1)
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What causes low 260/230 ratio for RNA & how to correct it - (reply: 14)
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RNA Storage - (reply: 1)
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Degrade contaminated DNA after RNA isolation? - (reply: 1)
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DNA ladder for total RNA integrity test? - (reply: 1)
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Both total RNA and mRNA in the same reaction? - (reply: 1)
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DNA extraction using RNAse (help needed) - (reply: 1)
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DNA extraction including RNAse - (reply: 1)
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DNA extraction after Total RNA isolation By Trizol - (reply: 2)
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Total RNA in RT-qPCR - (reply: 2)
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Air-dry isolated RNA? - (reply: 2)
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Total RNA extraction - problems (reply: 3)
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Does this make sense? REDISSOLVING THE RNA - (reply: 1)
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Isolation of total RNA and mRNA from human tissue - (reply: 5)
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Extraction total RNA - (reply: 1)
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RNA Isolation - Integrity of RNA (reply: 2)
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How to measure the concentration of RNA? - (reply: 4)
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RNA OD260/OD280 > 2, Why? - (reply: 3)
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DNA contamination in RNA extraction - (reply: 6)
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Working with DNA, RNA and oligonucleotides - (reply: 6)
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gels used for RNA detection - (reply: 3)
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Lyophilzed total RNA - (reply: 1)
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RNA isolation - purity (reply: 5)
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Determine the RNA concentraiton for linear PCR - (reply: 1)
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Buffer for red blood cell lysis for RNA extraction from blood - RNA methodology (reply: 6)
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Bioanalyser For RNA degradation, contradiction! - (reply: 1)
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Viral RNA & DNA extraction from serum - Protocols (reply: 1)
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big difference between two RNA quality test? - (reply: 2)
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How many is the limit? - What is the numerical limit to RNAi and others? (reply: 2)
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RNAse protection assay - No bands (reply: 2)
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RNA cleaning - (reply: 6)
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Question about silver staining - How silver saining can bind all of DNA, protein and RNA? (reply: 2)
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Problem with DNase treatment of RNA for RT-PCR - (reply: 1)
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Nuclear RNA knockdown with siRNA - is it possible? (reply: 6)
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internal standard for small RNA - (reply: 3)
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Difference between RNase A and RNase H - (reply: 1)
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Making TE buffer for RNA disolution - afraid of RNase contamination (reply: 3)
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RNAi in Jurkat cells - (reply: 3)
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Poor RNA quality - (reply: 3)
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trizol extraction for phospho proteins - (reply: 4)
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RNA Extraction using Trizol - How much Trizol should I use for rat brain tissue (reply: 5)
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RNA Isolation - (reply: 3)
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DNase digestion and RNA degradation - Cancer tissue RNA isolation (reply: 1)
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Need to improve RNA integrity - RNA Isolation (reply: 3)
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No RNA after TRIzol extraction - RNA isolation from mouse brain (reply: 6)
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gel analysis of large RNA products - (reply: 3)
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detection of alternatively spliced RNA by RTPCR or northern - detection of alternatively spliced RNA by RTPCR (reply: 2)
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RNA Isolation - (reply: 1)
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RNAi with macrophage - (reply: 1)
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Autoclave to kill RNases/DNases? - argument (reply: 6)
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RNA concentration - (reply: 3)
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about vectors and transfection of RNAi - (reply: 1)
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Isolating DNA and RNA from the same sample - (reply: 2)
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please recommend me a RNA isolation kit - RNA isolation from 100 tumor cells (reply: 1)
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RNA dimer - How to avoid RNA dimer formation (reply: 1)
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RNA extraction - RNA from 20yr old frozen breast tissue (reply: 1)
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RNAi vector for stable expression - (reply: 4)
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RT-PCR - double-strand RNA denaturation (reply: 2)
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RNA Isolation - (reply: 1)
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RNA Isolation from bacteria - (reply: 2)
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Transcription Rate - Measure Hn RNA using Taqman?? (reply: 1)
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RNA with DNA contamination - (reply: 1)
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DNA RNA extraction from adipose tissue - (reply: 2)
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RNA/protein Isolation - Is there some good KIT or ...? (reply: 3)
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RNA Extraction from Blood? - Need help with separating classes (reply: 1)
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Help for a friend - molecular biology, RNA DEGRADATION - (reply: 3)
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total rna extraction from whole blood - for rt pcr (reply: 3)
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RNA isolation from mouse blood - RNA for RT-PCR (reply: 1)
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RNA treatment - (reply: 2)
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RNA smells like bleach - (reply: 2)
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RNA isolation - Rna isolation (reply: 2)
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RNA from cultured Osteoblasts - osteoblasts (reply: 2)
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RNA extraction from snap frozen lung - (reply: 3)
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DNase treatment for RNA purification - (reply: 6)
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RNA and DNA extraction and PCR control - (reply: 1)
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Cell Cycle/DNA/RNA Unanswered Questions - test questions involving genetic info (reply: 2)
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RNA Isolation - (reply: 5)
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small RNAs extraction - need advices (reply: 1)
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cell culture RNA extraction - cell culture RNA extraction (reply: 4)
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RNA Interference - how to produce siRNA (reply: 10)
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RT-PCR, RNA isolation, etc. - (reply: 1)
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RNA extraction using Trizol - (reply: 4)
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rRNA in insects - Information about 18S, 5.8S, ITS1 & ITS2 (reply: 1)
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Treated for RNAses - (reply: 1)
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RNA transfer onto membrane - RNA (reply: 1)
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RNA electrophoresis - RNA electrophoresis (reply: 2)
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RNA contamination PCR - RNA contamination PCR (reply: 1)
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RNA isolation - RNA isolation from a single embryo (reply: 2)
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lymphocytes' RNA extraction - How many cells (reply: 1)
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RNA Extraction from ocular tissue - RNA Extraction from ocular tissue (reply: 2)
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Can RNA be extracted from paraffin-fixed tissues? - RNA extraction protocol for paraffin (reply: 1)
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RNA Structure Comparison - (reply: 2)
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Formaldehyde Gel - Question about RNA gel (reply: 1)
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DNA Contamination in RNA? - PCR of RNA (reply: 1)
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Help! DNA contamination in RNA and RT-PCR. - (reply: 1)
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RNA Degradation? - RNA Degradation? (reply: 1)
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PBMC RNA isolation - (reply: 1)
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which is more stable ineukaryotic cell,ssRNA or ds RNA - (reply: 1)
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I need some TRIZOL - (reply: 2)
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DIG RNA PROBES - (reply: 1)
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RNA in plasmid preparation interfere in ligation - RNA contamination and ligation (reply: 3)
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RNA isolation from bone - (reply: 1)
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improvment of high MW RNAs in northern blotting - (reply: 2)
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Isolation of RNA from freezedried - (reply: 1)
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RNA from whole blood - (reply: 2)
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Total RNA agaroseelectrophoresis - (reply: 1)
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RNA from Cocoa pod - (reply: 1)
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RNA long-term storage - (reply: 2)
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RNA isolation - (reply: 5)
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quantitative viral RNA assay - (reply: 1)
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RNA ISOLATION - (reply: 1)
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about extract RNA from spleen - (reply: 2)
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RNA electrophoresis - (reply: 1)
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assay for RNA helicase - (reply: 1)
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Difference between RNase Protention and RT-PCR - (reply: 1)
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How to isolate RNA from fungus? - (reply: 1)
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RNase and PCR - (reply: 1)
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non-radioactive successes out there? - non-radioactive RNA labelling that works please (reply: 1)
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How perform RNA extraction fromformalin-fixed tissues?? - (reply: 1)
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Isolating RNA for microarray - which kit (reply: 10)
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5ug polyA RNA, How to extractfrom mold form fungi? - (reply: 1)
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is hot weather a important factor in RNA extraction? - Does the degrade of Exracted RNA have a close relationship (reply: 1)
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Need to purify total RNA sample(contaminated with DNA) - (reply: 4)
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size of 18s rRNA and 28srRNA? - (reply: 1)
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Difference in RT PCR products when using total RNA or mRNA - (reply: 4)
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RNA quantitation from a veryminute quantity of starting material - (reply: 2)
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RNA isolation from a single cell - (reply: 1)
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RNA probe purification - (reply: 4)
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RNasing - (reply: 1)
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RNA prep/Northerns - (reply: 1)
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Removal of Rna from plasmid prep - (reply: 1)
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Isolation of total RNA from animal tissues and cells - by GuSCN and silico (reply: 1)
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RNA Newbie questions - (reply: 1)
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Measuring RNA concentration? - (reply: 1)
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Why is formaldehyde added to RNA gels? - (reply: 1)
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Re: RNA probe - (reply: 1)
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RNA isolation fromparaffin-embbed tissu - (reply: 1)
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Total RNA extraction from leaves - (reply: 1)
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RNA isolation from isolatedislets and RT-PCR - (reply: 1)
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RNA from PBMC - (reply: 1)
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RNA - Isolation - Which protocol is the likely best of RNA Isolation? (reply: 2)
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RNA & DNA extractionprotocols - (reply: 2)
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RNA isolation - (reply: 7)
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Spectrophotometric measurement of RNA quantity - In what should RNA be diluted for measurement at 260nm (reply: 2)
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A single band is observed afterthe isolation of RNA by kit...! - (reply: 1)
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RNase - (reply: 2)
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RNA isolation from PBMCs - (reply: 2)
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RNA on formaldehyde gel - (reply: 1)
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RNA ligation - (reply: 3)
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DNA contamination in RNA preparation - (reply: 1)
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Re: polysaccharides in RNApreps from plants - (reply: 2)
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Re: How to extract RNA fromFormalin fixed tissue - (reply: 7)
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RNA precipitation - (reply: 7)
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RNA extraction from cryopreserved bone marrow cells - RNA extraction method (reply: 3)
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DNA/RNA extraction from formalin fixed tissues - (reply: 1)