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total RNA isolatin - (Oct/26/2005 )

hi every body

i isolate total RNA from plant leaf by trizol.but when i runned RNA on 1% agarose gel ,i just got 1 band!
i dont know what is the resone.
is there anybody who can help me?
thanks a lot


what is the concentration? what is the 260/280 ratio?



good time.the concentration of the RNA is 0.88 micro gram /micoliter.and the 260/280 :1.601


Hi everybody:
I am also going to isolate RNA from a cultured cell. Seems Trizol is the most common kit for RNA isolation. Is that really good and the most cheap kit? unsure.gif