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Blood RNA extraction - Can the Qiagen RNeasy mini kit extract RNA from blood? (Apr/12/2007 )

Hi there,

I need to extract some RNA from blood. Can the regular Qiagen RNeasy mini kit do the job or do I HAVE to purchase the Qiagen kit that is designed for blood sample? I just need RNA that is good enough for regular PCR, so it doesn't have to be super high quality.

Thanks,
Cindy

-CindyToronto-

Hi Cindy,

we use qiazole first and then mix the aqueous phase wth the same amount of 70% ethanol. You can directly load the RNeasy column with that and proceed to the washing steps. Allways works good for me.

Krümel

-krümelmonster-

Hi Krumel,

Thx! Just wondering, does it work without qiazole, instead just use the lysate buffer with Beta-mercaptoethanol?

Cindy

QUOTE (krümelmonster @ Apr 13 2007, 04:40 AM)
Hi Cindy,

we use qiazole first and then mix the aqueous phase wth the same amount of 70% ethanol. You can directly load the RNeasy column with that and proceed to the washing steps. Allways works good for me.

Krümel

-CindyToronto-

In m hands, the 260:280 ratio allways was rather low...therefor I started using the Phenol step. K.

-krümelmonster-